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Biochemistry Laboratory
BIOL 3380
School of Natural Sciences and Mathematics
Current techniques in the purification and characterization of enzymes to demonstrate fundamental principles that are utilized in modern biochemistry and molecular biology research laboratories. Practical skills taught include micropipetting, basic solution preparation, conducting pH measurements, isolating crude enzyme extracts, and performing standard activity assays. Advanced experiments with Green Fluorescent Protein and Lactate Dehydrogenase include Ni++-NTA affinity chromatography, ion chromatography, protein detection using Bradford, Lowry, and spectrophotometric assays, SDS-PAGE separation, Western Blot analysis, and enzyme kinetics. Lab fee of $30 required. A Top Hat subscription fee of up to $40 is required for this course. Non-Enroll credit hours.
Prerequisite: BIOL 2281 or CHEM 2401 or equivalent.
Prerequisite or Corequisite: BIOL 3461 or CHEM 3461.
Offering Frequency: Each semester
Grades: 3,376
Median GPA: B
Mean GPA: 2.916
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Biochemistry Laboratory
BIOL 3380
School of Natural Sciences and Mathematics
Current techniques in the purification and characterization of enzymes to demonstrate fundamental principles that are utilized in modern biochemistry and molecular biology research laboratories. Practical skills taught include micropipetting, basic solution preparation, conducting pH measurements, isolating crude enzyme extracts, and performing standard activity assays. Advanced experiments with Green Fluorescent Protein and Lactate Dehydrogenase include Ni++-NTA affinity chromatography, ion chromatography, protein detection using Bradford, Lowry, and spectrophotometric assays, SDS-PAGE separation, Western Blot analysis, and enzyme kinetics. Lab fee of $30 required. A Top Hat subscription fee of up to $40 is required for this course. Non-Enroll credit hours.
Prerequisite: BIOL 2281 or CHEM 2401 or equivalent.
Prerequisite or Corequisite: BIOL 3461 or CHEM 3461.
Offering Frequency: Each semester
Grades: 3,376
Median GPA: B
Mean GPA: 2.916
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